| Elisabeth | Female | queen, sovereign/king | |
| Maria Stuart | Female | queen, sovereign/king | |
| Robert Dudley | Male | Landowner, nobility/aristocracy | |
| Georg Talbot | Male | Landowner, nobility/aristocracy | |
| Wilhelm Cecil | Male | nobility/aristocracy | |
| Graf von Kent | Male | Landowner, nobility/aristocracy | |
| Wilhelm Davidson | Male | Courtier | |
| Amias Paulet | Male | knight, Guard, uncle/aunt | |
| Mortimer | Male | nephew/niece | |
| Graf Aubespine | Male | nobility/aristocracy | |
| Graf Bellievre | Male | nobility/aristocracy | |
| Okelly | Male | friend | |
| Drugeon Drury | Male | knight, Guard | |
| Melvil | Male | Steward | |
| Hanna Kennedy | Female | nurse, servant | |
| Margaretha Kurl | Female | Chambermaid | |
| Scherif der Grafschaft | Male | law enforcement, officer | |
| Offizier der Leibwache | Male | officer | |
| Französische und Englische Herren | Male | extras, Gentleman | |
| Trabanten | Male | extras, Guard | |
| Hofdiener der Königin von England | Other | extras, servant, Courtier | |
| Diener und Dienerinnen der Königin von Schottland | Other | extras, servant, Courtier | |
